how to make normalization for your data in graphpad prism 8

No light-elicited necrosis was measured as compared to control cells maintained in darkness. Cell viability decrease was correlated to an increase in cell apoptosis indicated by caspase-3/7 activities in the same spectral range.

Irrespective of A2E concentration, the loss of cell viability was maximal for wavelengths from 415 to 455 nm.

Exposure to 10 nm illumination bands induced a loss in cell viability with a dose dependence upon A2E concentrations. Their absorption and emission spectra were similar to those of A2E. Retinal pigment epithelium cells incubated with A2E displayed fluorescent bodies within the cytoplasm. Six hours after light exposure, cell viability, necrosis and apoptosis were assessed using the Apotox-Glo Triplex™ assay. Light irradiances were normalized with respect to the natural sunlight reaching the retina. Primary cultures of porcine retinal pigment epithelium cells were incubated for 6 hours with different A2E concentrations and exposed for 18 hours to 10 nm illumination bands centered from 380 to 520 nm in 10 nm increments. Our aim was to define the most toxic wavelengths in the blue-green range on an in vitro model of the disease. The high energy visible light between 380 nm and 500 nm (blue light) is incriminated. A photosensitive derivative of the visual pigment, N-retinylidene- N-retinylethanolamine (A2E), may be involved in this phototoxicity. Among the identified risk factors of age-related macular degeneration, sunlight is known to induce cumulative damage to the retina.

How to make normalization for your data in graphpad prism 8